Sterile preparation of antibiotic-selective LB agar plates using a microwave oven.

نویسندگان

  • M P Iacoviello
  • S A Rubin
چکیده

WI-L2-NS cells, a p53 mutant cell line. Mutat. Res. 352:79-86. 2.Cherbonnel-Lasserre, C., S. Gauny, and A. Kronenberg. 1996. Suppression of apoptosis by Bcl-2 or Bcl-xL promotes susceptibility to mutagenesis. Oncogene 13:1489-1497. 3.Dignam, J.D., R.M. Lebovitz, and R.G. Roeder. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11:1475-1489. 4.Harlow, E. and D. Lane. 1999. Using Antibodies: A Laboratory Manual. CSH Laboratory Press, Cold Spring Harbor, NY. 5.Little, J.B., H. Nagasawa, P.C. Keng, Y. Yu, and C.Y. Li. 1995. Absence of radiation-induced G1 arrest in two closely related human lymphoblast cell lines that differ in p53 status. J. Biol. Chem. 270:11033-11036. 6.Stürzbecher H.W., B. Donzelmann, W. Hennig, U. Knippschild and S. Buchhop. 1996. p53 is linked directly to homologous recombination processes via RAD51/RecA protein interaction. EMBO J. 15:1992-2002. 7.Timmons, T.M. and B.S. Dunbar. 1990. Protein blotting and immunodetection, p. 679688. In M.P. Deuscher (Ed.), Methods in Enzymology, Vol. 182. Guide to Protein Purification. Academic Press, San Diego. 8.Xia, F., X. Wang, Y.H. Wang, N.M. Tsang, D.W. Yandell, K.T. Kelsey, and H.L. Liber. 1995. Altered p53 status correlates with differences in sensitivity to radiation-induced mutation and apoptosis in two closely related human lymphoblast lines. Cancer Res. 55:12-15. 9.Zhen, W., C.M. Denault, K. Loviscek, S. Walter, L. Geng, and A.T. Vaughan. 1995. The relative radiosensitivity of TK6 and WIL2-NS lymphoblastoid cells derived from a common source is primarily determined by their p53 mutational status. Mutat. Res. 346:85-92.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Increased genome instability in Escherichia coli lon mutants: relation to emergence of multiple-antibiotic-resistant (Mar) mutants caused by insertion sequence elements and large tandem genomic amplifications.

Thirteen spontaneous multiple-antibiotic-resistant (Mar) mutants of Escherichia coli AG100 were isolated on Luria-Bertani (LB) agar in the presence of tetracycline (4 microg/ml). The phenotype was linked to insertion sequence (IS) insertions in marR or acrR or unstable large tandem genomic amplifications which included acrAB and which were bordered by IS3 or IS5 sequences. Five different lon mu...

متن کامل

Microwave oven in microbiology.

Editor, Reference to Microwave oven in microbiology laboratory, JPMA., 1992:42:2. In response to the queries by a microbiologist regarding the published paper we feel it would be appropriate to clarifr the facts. 1. Sterilization in microwaves works on a different principle than the conventional autoclave and sterilizadon is independent of temperature, just like the principle of sterilization b...

متن کامل

Machine for automatic bacteriological pour plate preparation.

A fully automatic system for preparing poured plates for bacteriological analyses has been constructed and tested. The machine can make decimal dilutions of bacterial suspensions, dispense measured amounts into petri dishes, add molten agar, mix the dish contents, and label the dishes with sample and dilution numbers at the rate of 2,000 dishes per 8-hr day. In addition, the machine can be prog...

متن کامل

Preparation of agar wells for antibiotic assay.

A variety of devices such as stainless-steel or ceramic cylinders, single or multiple in design, and paper discs of several sizes are commonly used for antibiotic assays performed by the agar diffusion method. Holes cut in agar (agar wells) are wells with melted agar is often necessary to obtain uniform inhibition zones (2). This note describes a method for the preparation of agar-well plates w...

متن کامل

Copacabana method for spreading E. coli and yeast colonies.

Molecular biology techniques require the isolation of individual clones for analysis after bacterial or yeast transformation. For E. coli and Saccharomyces cerevisiae, the dispersion of colonies on plates has traditionally involved the use of a glass spreader—a bent glass rod that could be repeatedly flame sterilized and used to spread a small aliquot of microorganisms from the center of the pl...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • BioTechniques

دوره 30 5  شماره 

صفحات  -

تاریخ انتشار 2001